Researchers evaluated the impact of chewing on bones on the dental roots, enamel, and gingiva of 12 Beagle dogs. The dogs were randomly divided into 2 treatment groups, six were given a cortical bone (CB) and six were given a spongy bone (SB). Intra-oral radiographs were obtained on days 0 and 14, and calculus assessment was performed using images captured on days 0, 3, 6, 9, 12, and 14.

Dental calculus was completely removed from the first and second premolars and molars within 3 days of supplementation. Bones were highly effective for calculus removal and gingival inflammation reduction.

Despite the hardness of bones, no lesions or teeth root and enamel fracture, or esophageal or intestinal obstructions-complications related to bone ingestion-were noted. However, spongy bones showed some gingival lesions and bone remnants between teeth. Gingival lesions were caused by the daily and continuous supply of new pieces of bone for 13 days.

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Evaluation of teeth injuries in Beagle dogs caused by autoclaved beef bones used as a chewing item to remove dental calculus.