Diagnosing Canine Hepatozoonosis
Texas A&M Veterinary Medical Diagnostic Laboratory (TVMDL) diagnosed canine hepatozoonosis in a muscle biopsy from a 3-year-old dachshund-mix dog. The patient was reported to have non-specific clinical signs with a neutrophilic leukocytosis and a WBC of 50,000. The submitting veterinarian sent formalin-fixed punch biopsies from the triceps and semitendinosus muscles to TVMDL. Histopathology revealed a mild granulomatous myositis with scattered onion-skin cysts characteristic of H. americanum.
These parasites are often widely distributed throughout all skeletal muscle. Since they can be scattered through the tissue, submission of multiple biopsy specimens is recommended to increase the chance of collecting a diagnostic sample. Histologically, organisms can be found in skeletal and cardiac muscle and may be accompanied by pyogranulomatous inflammation. The parasites are often surrounded by multiple layers of mucopolysaccharide, creating a characteristic “onion-skin” cyst,
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Canine hepatozoonosis diagnosed via skeletal muscle biopsy